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A comparative account of primer designing resources, feature-validations and application-based categorizations

Aceprimer

Tool nameAceprimer
URLhttp://elegans.bcgsc.bc.ca/aceprimer/aceprimer.shtml
Important features1. Allows the user to design primers for C. elegans genome. 2. Allows the user to search for deletion alleles in Caenorhabditis elegans gene.
CitationsMcKay SJ, Jones SJ. AcePrimer: automation of PCR primer design based on gene structure. Bioinformatics. 2002 Nov;18(11):1538-9. PubMed PMID: 12424127.
Year of publication2002
Rank by usage frequency100
Comments
FunctionReal time PCR, General PCR, Primer specificity checking
CategoryOnline, Free
Features1. Allows the user to design poison, nested and RT PCR primers. 2. Designs PCR primers to search for deletion alleles in C.elegans gene and also design primers for nested PCR. 3. Has an option to specify the region and chromosome number to design primers. 4. Designs primers for each exons and displays the graphical view of it. 5. Allows the user to check the specificity of primer pairs against the entire C.elegans genome. 6. Displays the transcript variants of target gene graphically.
DescriptionFor amplification and expression of specific splice variants.

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