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A comparative account of primer designing resources, feature-validations and application-based categorizations


Tool nameAutoPrime
Important features1. Designs RT-PCR primers. 2. Allows the user to design primers to the exon-exon border sequence.
CitationsGunnar Wrobel1, Felix Kokocinski, and Peter Lichter. AutoPrime: selecting primers for expressed sequences. Genome Biology 2004.
Year of publication2004
Rank by usage frequency100
CommentsDid not give results when tested [as per 5th july, 2011].
FunctionJunction primers
CategoryOnline, Free
Features1. Autoprime takes ensembl genome browser data only. 2. It refers to the mispriming library, repbase. 3. Allows the user to design primers specific to mRNA sequence and non-specific to genomic DNA sequence. 4. It is based on primer 3 software.
DescriptionFor methylation pattern study of CpG island; eventually understanding the gene expression pattern.

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