Alternative splice form analysis

BLAT - BLAST like Alignment tool
http://genome.ucsc.edu/cgi-bin/hgBlat?command=start

Kent WJ. BLAT--the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64. PubMed PMID: 11932250; PubMed Central PMCID: PMC187518.

2002

1) Link to UCSC Genome browser is given. 2) Splice Sites are marked in the sequence itself, with light blue colour.

ASAP II - Alternative Splicing Annotation Project
http://bioinfo.mbi.ucla.edu/ASAP2/

Kim N, Alekseyenko AV, Roy M, Lee C. The ASAP II database: analysis and comparative genomics of alternative splicing in 15 animal species. Nucleic AcIDs Res. 2007 Jan;35(Database issue):D93-8. Epub 2006 Nov 15. PubMed PMID: 17108355; PubMed Central PMCID: PMC1669709.

2006

100

1) Gives Genomic expression. 2) Gives Genomic location, gene aliases etc. as gene information. 3) Orthologous genes identified by MULTIZ alignments are given. 4) Isoform sequences are given. 5) Type of exons- Alternative or Constitute are given along with genomic position and number of EST and mRNA observed. 6) Intron type- Alternative or Constitutive, 5 site, 3 site, whether canonical or not and splice type, all information is provided. 7) Alternative Splicing information is provided.

ASPic - Alternative Splicing Prediction
http://t.caspur.it/ASPIC/index.php

1) Detect the exon-intron structure of a gene. 2) Predicts constitutive and alternative splice sites.

Bonizzoni P, Rizzi R, Pesole G. ASPIC: a novel method to predict the exon-intron structure of a gene that is optimally compatible to a set of transcript sequences. BMC Bioinformatics. 2005 Oct 5;6:244. PubMed PMID:16207377; PubMed Central PMCID: PMC1276783.

2005

100

1) Requires registration. 2) Gene name Brca1 and mybl2 as well as uploaded sequence didn't receive any results when used in the option of 'RUN Aspic'. 3) Provides option of retrieving Unigene ID for input. 4) Results are obtained in E-mail. 5) Gives genomic and transcript sequences. 6) Gene structure view is provided, showing Refseq exon, Novel exon, Refseq Intron, Novel intron, Fuzzy Intron, Canonical splice site and Non-Canonical splice site. 7) Shows 3'UTR and 5'UTR, Poly A site 8) Splice si

ASPicDB - Alterenative Splicing Prediction database
http://t.caspur.it/ASPicDB/

Castrignan� T, D'Antonio M, Anselmo A, Carrabino D, D'Onorio De Meo A, D'Erchia AM, Licciulli F, Mangiulli M, Mignone F, Pavesi G, Picardi E, Riva A, Rizzi R, Bonizzoni P, Pesole G. ASPicDB: a database resource for alternative splicing analysis. Bioinformatics. 2008 May 15;24(10):1300-4. Epub 2008 Apr 3. PubMed PMID: 18388144.

2008

100

Astalavista - Alternative splicing transcriptional landscape visualization tool
http://genome.crg.es/astalavista/

Identifies complex Alternative Splice events by comparing all given transcripts.

Foissac S, Sammeth M. ASTALAVISTA: dynamic and flexible analysis of alternative splicing events in custom gene datasets. Nucleic AcIDs Res. 2007 Jul;35(Web Server issue):W297-9. Epub 2007 May 7. PubMed PMID: 17485470; PubMed Central PMCID: PMC1933205.

2007

100

1) The results can be browsed on UCSC browser. 2)Results can be downloaded in GTF or ASTA format. 3) Splice site details include the chromosome number, and the position of Alternative Splice sites. 4) Intron- Exon structure is also shown. 5) Types of Alternative Splice Events include exon skipping, alt acceptor and intron retention.

AVATAR
http://avatar.iecs.fcu.edu.tw/

Hsu FR, Chang HY, Lin YL, Tsai YT, Peng HL, Chen YT, Cheng CY, Shih MY, Liu CH, Chen CF. AVATAR: a database for genome-wIDe alternative splicing event detection using large scale ESTs and mRNAs. Bioinformation. 2005 Apr 22;1(1):16-8. PubMed PMID: 17597845; PubMed Central PMCID: PMC1891626.

2005

100

URL not working as on 14 Dec 2012

H-DBAS - Human Transcriptome Database for Alternative Splicing
http://jbirc.jbic.or.jp/h-dbas/

Takeda J, Suzuki Y, Sakate R, Sato Y, Gojobori T, Imanishi T, Sugano S. H-DBAS: human-transcriptome database for alternative splicing: update 2010. Nucleic AcIDs Res. 2010 Jan;38(Database issue):D86-90. Epub 2009 Dec 7. PubMed PMID: 19969536; PubMed Central PMCID: PMC2808982.

2010

100

1) Gene information gives chromosome number, position and strand. 2) Results can be downloaded. 3) Splice site details are shown graphically. 4) Also shows SNP in the graph. 5) Gene expression information is given, giving tissue specific expression in Affymetrix gene chip and Agilent. 6) Also gives information about Transcript feature.